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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants have been reported but in-depth nucleotide sequence data is not still obtainable.

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Blocking peptides are peptides that bind specifically for the target antibody and block antibody binding. These peptide normally consists of the epitope acknowledged from the antibody.

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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best final results are...

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This information outlines even further doable optimization of mobile and immunoassay parameters to be certain the best possible effects are...

Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.

Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose members are characterised by a dramatic periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of every mitotic party. Cyclin E forms a complex with and features like a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates at the G1-S stage boundary and is read more particularly degraded as cells progress through S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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